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|Title:||A gut reaction: effects of an e. coli-produced toxin on gut hormone regulation and gut physiology|
|Keywords:||Escherichia coli;ETEC;toxins;proteins;hormones;glucagon-like peptide-2;hormone secretion;enterotoxins;gastrointestinal;enterotoxigenic;enteroendocrine|
|Abstract:||Enterotoxigenic Escherichia coli (ETEC) produces the heat-stable enterotoxin b (STb), which is responsible for secretory diarrhea in humans and animals. STb disrupts intestinal fluid homeostasis, epithelial barrier function, and promotes apoptosis. Glucagon-like peptide-2 (GLP2) is an intestinotrophic growth hormone secreted by enteroendocrine L-cells which enhances cellular proliferation, epithelial barrier function, and inhibits apoptosis. As many similarities are clear between GLP-2 production and secretion pathways and STb cell signaling mechanisms, this study aims to investigate the effects STb has on the physiology of in-vitro L-cells and the secretion of GLP-2. Enteroendocrine cells which secreted the highest amounts of growth hormone were found to be more resistant to STb intoxication. STb was found to enhance the secretion of GLP-2, potentially due to similar cellular mechanisms of hormone production and secretion and STb cell signaling. GLP-2 was found to inhibit the toxigenic effects of STb. Future work will elucidate the mechanism of this interaction. This work will support the use of GLP-2 therapies in enterotoxigenic related diseases.|
|Appears in Collections:||Biology - Master's Theses|
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|Shahn Butt Thesis Final Submission.pdf||1.72 MB||Adobe PDF||View/Open|
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