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Title: Protein-patterned EMF as enzymatic moderators: evidence for a non-invasive method of cellular manipulation
Authors: Plourde-Kelly, Adam
Keywords: Electromagnetic fields;PC-12 cells;planaria;differentiation;regeneration;p38, ERK;resonant recognition model
Issue Date: 16-Sep-2022
Abstract: The resonant recognition model states that each protein can be classified by function through a characteristic frequency obtained based on the electrical signature of the whole protein. This electrical signature reflects the accumulation of specific energy values from each of the 20 amino acids comprising bioproteins. A litany of evidence suggests that exposure to complex electromagnetic fields (EMF) can influence functioning of biological systems. We therefore postulated that exposure to a magnetic field patterned after the specific electrical signature of a protein, calculated using the resonant recognition model would be sufficient to target and resonate with the modeled protein. Therefore, we created EMF patterns from the amino acid sequence of two different signaling molecules, p38 and ERK, using the resonance recognition model. We exposed PC-12 cells to p38-patterned EMF using two different geometries and measured its ability to affect the differentiation of the cells in vitro. We found that exposure to the p38 EMF for three days significantly increased the proportion of differentiated PC-12 cells (t=-2.515, p=.027). The effect of the ERK-patterned EMF on planarian regeneration was examined in a subsequent in vivo experiment. We found that exposure of bisected planaria to a sine field caused significantly greater regeneration at 7 days compared to exposure to the ERK-patterned EMF (U=637, W=1540, Z=-1.977, p=0.048). The results demonstrate the potential of protein-patterned EMF in modulating biological processes. We propose that there may be both specific and non-specific effects that result from exposure to protein-patterned EMF that can interact with one another for constructive or destructive effects. The results of this study are robust and demand further study on the mechanistic function of these fields.
Appears in Collections:Biology - Master's Theses

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