Please use this identifier to cite or link to this item: https://zone.biblio.laurentian.ca/handle/10219/3826
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dc.contributor.authorAlharbi, Albatul-
dc.date.accessioned2022-01-27T15:35:47Z-
dc.date.available2022-01-27T15:35:47Z-
dc.date.issued2021-12-16-
dc.identifier.urihttps://zone.biblio.laurentian.ca/handle/10219/3826-
dc.description.abstractIn traditional medicine, Ferula assafoetida (F. assafoetida), has been used as an antiseptic, anti-diabetic, anti-inflammatory, and anti-cancer agent. In recent years its anti-cancer and antiinflammatory activities have become a focus in drug research. We investigated the in vitro cytotoxicity and anti-inflammatory effects of ethanolic extracts of F. assafoetida and five known components (ferulic acid, vanillic acid, quercetin, ellagic acid, and p-coumaric acid) on a group of malignant and non-malignant breast cell lines and the THP-1 monocyte-like cell line. Our results showed that treatment with the ethanolic extract of F. assafoetida, and the components, had a significant effect on cell viability and apoptosis induction for the human MCF-7, MDA-MB-231, and murine 4T1 breast cancer cell lines compared to the non-malignant human HBL-100 breast cells. This research also showed that THP-1 peripheral blood monocytic leukemia cells, differentiated into macrophages, could be further polarized into the M1 inflammatory phenotype by treatment with extracts of F. assafoetida and the components. There was a significant increase in the expression of CD80, a marker associated with the M1 macrophage subtype, but no increase in expression of the M2 subtype marker, CD163, in treated cells. Further, this polarization of the THP-1-dependent macrophages showed an increased ability to damage MCF-7 or MDA-MB-231 cell monolayers in co-culture experiments. Therefore, treatment with F. assafoetida extracts can also indirectly cause the death of cancer cells via activation of immune cells. These results confirm that F. assafoetida is a potential source of anti-cancer and immune modulatory compounds and that further investigation is needed to reveal the mechanisms of F. assafoetida’s effects on apoptosis and immunomodulation.en_US
dc.language.isoenen_US
dc.subjectFerula assafoetida,en_US
dc.subjectanti-cancer,en_US
dc.subjectpro-inflammation,en_US
dc.subjectcytotoxicityen_US
dc.subjectapoptosisen_US
dc.subjectimmunomodulationen_US
dc.subjectmacrophage polarizationen_US
dc.titleCellular effects of Ferula Assafoetida on breast cancer cells and inflammatory responses in cultured monocytesen_US
dc.typeThesisen_US
dc.description.degreeDoctor of Philosophy (PhD) in Biomolecular Sciencesen_US
dc.publisher.grantorLaurentian University of Sudburyen_US
Appears in Collections:Biomolecular Sciences - Doctoral Theses

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