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DC Field | Value | Language |
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dc.contributor.author | Williams, Laura Marie | - |
dc.date.accessioned | 2021-12-10T20:56:46Z | - |
dc.date.available | 2021-12-10T20:56:46Z | - |
dc.date.issued | 2020-08-24 | - |
dc.identifier.uri | https://zone.biblio.laurentian.ca/handle/10219/3794 | - |
dc.description.abstract | Glucagon-like peptide-1 (GLP-1) is an insulin-stimulating hormone released from enteroendocrine cells. Muramyl dipeptide (MDP) is a peptidoglycan motif which has insulin- sensitizing effects in obesogenic mice by acting through the nucleotide oligomerization domain 2 (NOD2) receptor. We hypothesized that MDP enhances glucose tolerance by inducing intestinal GLP-1 secretion through NOD2 activation. We observed a significant increase in GLP-1 secretion when L-cells were treated with a fatty acid MDP derivative (L18-MDP). Additionally, we demonstrated NOD2 expression in mouse intestine and in L-cells. Two intraperitoneal injections of MDP (5mg/kg) significantly increased fasting total GLP-1 in chow-fed mice; an effect that was lost during the onset of hyperglycemia during a high-fat diet. No improvement in oral glucose tolerance was observed in MDP-treated mice. Finally, we demonstrated in L-cells that hyperglycemic conditions reduce NOD2 and GLP-1 mRNA expression. Together these findings suggest MDP may play a role in enhancing GLP-1 during euglycemia but loses its ability to do so in hyperglycemia. | en_US |
dc.language.iso | en | en_US |
dc.subject | Muramyl dipeptide | en_US |
dc.subject | glucagon-like peptide-1 | en_US |
dc.subject | Type 2 Diabetes | en_US |
dc.subject | NOD2 | en_US |
dc.subject | oral glucose tolerance | en_US |
dc.title | High-fat diet-induced loss of muramyl dipeptide sensitivity | en_US |
dc.type | Thesis | en_US |
dc.description.degree | Master of Science (MSc) in Biology | en_US |
dc.publisher.grantor | Laurentian University of Sudbury | en_US |
Appears in Collections: | Biology - Master's Theses Master's Theses |
Files in This Item:
File | Description | Size | Format | |
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Laura Williams MSc thesis July 13th.pdf | 1.73 MB | Adobe PDF | View/Open |
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