Please use this identifier to cite or link to this item:
https://zone.biblio.laurentian.ca/handle/10219/3744
Title: | Glutamine synthetase expression and stress tolerance in Drosophila melanogaster |
Authors: | Daypuk, Jenna |
Keywords: | Glutamine synthetase;glutamine addiction;oxidative stress;heat shock;Drosophila melanogaster |
Issue Date: | 3-Aug-2021 |
Abstract: | This thesis explores glutamine synthetase in Drosophila melanogaster. L-glutamine, more than other amino acids, is strictly required for cell survival. Like many complex organisms, D. melanogaster has two forms of the enzyme glutamine synthetase (GS): GS1, located in the mitochondria, and GS2, located in the cytosol. Interestingly, how these two isozymes partition GS function is unknown. I validated a sample preparation protocol to separate the mitochondria and cytosol from Drosophila samples, and assay solutions for the measurement of GS transferase and GS biosynthetic activity. I created knockdowns of GS1 and GS2 and subjected these to heat shock and paraquat-induced stress. The GS1 and GS2 knockdowns displayed differences in their response to both stressors, indicating a potential functional differentiation of the two isozymes in stress tolerance. This project is a step in the use of Drosophila to explore the GS network and how glutamine modulates cell survival. |
URI: | https://zone.biblio.laurentian.ca/handle/10219/3744 |
Appears in Collections: | Chemical Sciences - Master's Theses Master's Theses |
Files in This Item:
File | Description | Size | Format | |
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_Jenna Daypuk Masters thesis 080621.pdf | 8.59 MB | Adobe PDF | View/Open |
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