Please use this identifier to cite or link to this item: https://zone.biblio.laurentian.ca/handle/10219/3566
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dc.contributor.authorAlshehri, Amal-
dc.date.accessioned2020-09-28T15:18:48Z-
dc.date.available2020-09-28T15:18:48Z-
dc.date.issued2020-08-13-
dc.identifier.urihttps://zone.biblio.laurentian.ca/handle/10219/3566-
dc.description.abstractType 2 diabetes (T2D) accounts for 90% of all cases of diabetes, and it is one of the world’s most threatening chronic and metabolic diseases in the world. Researchers have been investigating the possible links between the glucagon-like peptide1 (GLP-1) incretin hormone and bacterial metabolites in the gut in order to improve T2D treatments. In this study, I hypothesized that muramyl dipeptide (MDP) enhances the release of GLP-1 to improve hyperglycemia. Various muropeptides were tested in GLP-1 secreting cell lines (NCI-H716 and GLUTag) for their ability to regulate hormone secretion. The intracellular mechanism of lipid-modified L18MDP was examined via western blot and pharmacological inhibition. Pilot studies using siRNA for the MDP receptor NOD2 were completed. GLP-1 secretion was significantly induced by different concentrations of L18MDP in the two cell lines, (5, 10 µg/ml) in GLUTag and (10 µg/ml) in NCIH716. Treatment did not affect cell lines viability. The p38 MAPK signaling pathway was slightly stimulated at the 10-minute L18MDP treatment as was demonstrated by western blot. Pharmacological NOD2 inhibition with GSK717 at different doses (5, 10 µM) failed to block the L18MDP stimulation of GLP-1 secretion in GLUTag and NCI-H716 cell lines. This work demonstrates that L18MDP can significantly stimulate GLP-1 release; however, future work will be required to resolve the precise mechanismen_US
dc.language.isoenen_US
dc.subjectGlucagon-like peptide 1en_US
dc.subjectGPL-1en_US
dc.subjectmuramyl dipeptideen_US
dc.subjectMDPen_US
dc.subjectL18MDPen_US
dc.subjectp38 MAPKen_US
dc.titleThe role of muramyl depeptide in glucagon-like peptide-1 regulationen_US
dc.typeThesisen_US
dc.description.degreeMaster of Science (MSc) in Biologyen_US
dc.publisher.grantorLaurentian University of Sudburyen_US
Appears in Collections:Biology - Master's Theses
Master's Theses

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