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dc.contributor.authorHulley, Emily-
dc.description.abstractReliable species identification methods are important for industrial environmental monitoring programs. Probe based real-time PCR (qPCR) provides an accurate, cost-effective and high-throughput method for species identification. Here we present the development and validation of species-specific primers and probe for the identification of eight freshwater fish species. The development of a fully automated species-decoder algorithm allowed for target species identification with 100% accuracy while completely removing any false-positive detection of non-target species. Furthermore, the probe-based qPCR technique utilized in this study is substantially more cost-effective and time efficient than DNA barcoding and morphological identification methods. The qPCR assays were also highly sensitive and accurately detected target species from collected environmental DNA (eDNA) samples. In summary, probe-based multiplex qPCR assays provide a rapid and accurate method for freshwater fish species identification and the methodology established in this study can be utilized for various other species identification initiatives.en_CA
dc.subjectfreshwater fish speciesen_CA
dc.subjectenvironmental monitoringen_CA
dc.subjectcytochrome oxidase Ien_CA
dc.subjectDNA barcodingen_CA
dc.subjectmorphological identificationen_CA
dc.subjectTaqMan real-time PCRen_CA
dc.subjectenvironmental DNAen_CA
dc.subjectnext generation sequencingen_CA
dc.subjectspecies detectionen_CA
dc.subjectspecies identificationen_CA
dc.titleMolecular techniques for the identification of freshwater fish species for environmental monitoring programsen_CA
dc.description.degreeMaster of Science (MSc) in Biologyen_CA
dc.publisher.grantorLaurentian University of Sudburyen_CA
Appears in Collections:Biology - Master's Theses
Master's Theses

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