Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPichette, Jennifer-
dc.description.abstractMetabolic hormones released from the gastrointestinal tract are core regulators for digestion, satiety, gut motility, and glucose homeostasis. Advancements in the management of type 2 diabetes and obesity have recently included the use of incretin hormone-based therapies, such as glucagon-like peptide-1 (GLP-1) analogs. GLP-1 can enhance glucose-dependent insulin secretion and stimulate satiety after a meal. The release of this incretin is stimulated by the entry of nutrients in the lumen or circulation. In addition, microbial metabolites have recently emerged as a new group of potent stimulators of GLP-1 secretion. Hydrogen sulfide (H2S), produced from sulfate-reducing bacteria (SRB) of the distal ileum and colon, are localized in the same niche as GLP-1 secreting L-cells. The physiological role of endogenous H2S is well established in many biological systems, however it is not well understood how or if H2S can affect the enteroendocrine system. Therefore, we hypothesized that microbial H2S from the distal GI tract can potentially alter GLP-1 secretion and downstream metabolism, and further investigated the potential mechanisms involved in the process. In murine colonic L-cells (GLUTag), we demonstrated that H2S donors (NaHS and GYY4137) significantly enhanced GLP-1 secretion and that the process was regulated through the p38 MAPK signaling pathway. In male C57BL/6 mice, a 4-week chondroitin sulfate prebiotic diet successfully elevated SRB and colonic H2S levels, enhanced the GLP-1 response, and reduced food intake. Together, this study demonstrated a direct role for H2S in the stimulation of GLP-1 and a potential role for sulfur prebiotics to increase the H2S producing SRB as a means to enhance GLP-1 and improve metabolismen_CA
dc.subjectgut microbiomeen_CA
dc.subjectglucagon-like peptide-1en_CA
dc.titleThe role of intestinal hydrogen sulfide on GLP-1 secretion and downstream metabolismen_CA
dc.description.degreeMaster of Science (MSc) in Biologyen_CA
dc.publisher.grantorLaurentian University of Sudburyen_CA
Appears in Collections:Biology - Master's Theses
Master's Theses

Files in This Item:
File Description SizeFormat 
Jpichette Final revised thesis to Grad studies.pdf853.82 kBAdobe PDFThumbnail

Items in LU|ZONE|UL are protected by copyright, with all rights reserved, unless otherwise indicated.