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dc.contributor.authorHunt, Sarah C.-
dc.description.abstractRBM5 is a tumour suppressor gene with substantially decreased expression in most lung cancers, especially in smokers, that may result from gene deletion. The objective of this study was to determine if significantly decreased levels of RBM5 expression in the lung cancers of smokers was related to RBM5 deletion. Using DNA from patient lung tissue, RBM5 gene copy number was quantified by quantitative polymerase chain reaction. Although deletions were detected in the lung specimens, RBM5 gene copy number was not significantly decreased in lung tumours. RBM5 deletions were detected in the non-tumour DNA of smokers, but not in the never-smokers, indicating that RBM5 gene deletion might be related to smoking. In conclusion, it appeared as though RBM5 was downregulated by more than one mechanism, which included gene deletion. Further analyses must be carried out to examine other mechanisms by which RBM5 is downregulated.en_CA
dc.subjectnon-small cell lung canceren_CA
dc.subjectgene copy numberen_CA
dc.subjectgene deletionen_CA
dc.subjecttobacco smoke exposureen_CA
dc.titleExamination of gene deletion as a mechanism of RBM5 downregulation in tobacco smoke-associated lung canceren_CA
dc.description.degreeMaster of Science (MSc) in Chemical Sciencesen_CA
dc.publisher.grantorLaurentian University of Sudburyen_CA
Appears in Collections:Chemical Sciences - Master's Theses
Master's Theses

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