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|Title:||Chronic nitrogen and nutrient deposition impacts on community structure and abundance of bacteria, fungi, and CH4 cycling prokaryotes in a northern peat bog|
|Keywords:||Peatland;Mer Bleue;nitrogen deposition;microbial community|
|Abstract:||Anthropogenic nitrogen (N) deposition may threaten peatland carbon storage capabilities. Increased deposition has been linked to subsequent plant community shifts and increased decomposition rates, potentially via the disruption of natural microbial communities. By examining peat soils from randomized, replicated treatment plots in a long-term simulated chronic N deposition experiment at the Mer Bleue Bog in Eastern Ontario, Canada, my objective was to characterize how increased deposition impacts community structure and abundance of broad groups of microbial communities, and specific CH4 cycling prokaryotes. Using fingerprinting approaches and qPCR of SSU rRNA and other functional genes, my data show that with increasing nutrient loading the bacterial and fungal community structure changed. Along the same gradient methanogen abundance decreased, however there were no corresponding changes in methanotroph community structure or abundance. My results provide new insights on the possible causes of higher CO2 and CH4 effluxes seen in situ following chronic nutrient loading.|
|Appears in Collections:||Master's Theses|
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