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https://zone.biblio.laurentian.ca/handle/10219/2399
Titre: | Intramuscular immunization of mice with KatA (catalase) generates functional antibodies against Campylobacter jejuni invasion of gut epithelial cells in vitro |
Auteurs: | Rickaby, Barbara Ashley |
Mots clés: | Campylobacter jejuni;catalase;functional antibodies;KatA;vaccine, |
Date publié: | 27-mai-2015 |
Éditeur: | Laurentian University of Sudbury |
Abstrait: | The high incidence of Campylobacter jejuni-associated diarrhea, increase in the frequency of drug resistance in clinical isolates, and the recent association of C. jejuni infections and Guillain-Barré syndrome has heightened the need to develop effective anti-Campylobacter vaccines. Due to the risk of auto-immunity, vaccination using inactivated whole-cells or attenuated cells cannot be considered. Subunit vaccines however are a viable alternative and have been shown to be effective against similar pathogens. The main goal of this research is to test efficacy of the catalase A (KatA) protein as vaccine candidate against this pathogen. It was determined that KatA co-fractionates with both the soluble and insoluble cell fractions and a balanced IgG1/IgG2a response could be produced against it following immunization in mice. It was demonstrated that these antibodies can mediate complement-specific bactericidal activity and reduced adhesion and invasion of human epithelial (Caco-2) cells by C. jejuni using established in vitro assays. |
URI: | https://zone.biblio.laurentian.ca/dspace/handle/10219/2399 |
Apparaît dans les collections: | Biology - Master's Theses Master's Theses |
Fichiers dans cet item:
Fichier | Description | Taille | Format | |
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M Sc Thesis B Rickaby 2015_1.pdf | 815.82 kB | Adobe PDF | Parcourir/Ouvrir |
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