Please use this identifier to cite or link to this item:
https://zone.biblio.laurentian.ca/handle/10219/2227
Title: | Examining the distribution of ketamine and metabolites in decomposed skeletal tissues: development of a high throughput approach and application to an environmental and decomposition study |
Authors: | Cornthwaite, Heather |
Keywords: | Forensic Toxicology;Bone;Ketamine;GC-MS |
Issue Date: | 7-Aug-2014 |
Publisher: | Laurentian University of Sudbury |
Abstract: | The use of skeletal tissues in forensic toxicology research has increased dramatically in the last 5 years. These studies have yielded valuable information pertinent to using skeletal tissues as a biological matrix in drug analysis. The majority of these studies have been completed on a small scale so a larger scale is needed for further research. Here, a high throughput microwave assisted extraction (MAE) and microplate solid phase extraction (MPSPE) protocol is developed and implemented to examine how body position and microclimate affect ketamine and metabolite distribution in decomposed skeletal tissue. Analytes were successful recovered in 30 min following MAE compared to 180 min for passive solvent extraction (PSE). Body proved to be significantly different in the observed drug levels at both microclimates. The sunlit microclimate had significantly higher drug levels, with less maggot activity, compared to the shaded microclimate. |
URI: | https://zone.biblio.laurentian.ca/dspace/handle/10219/2227 |
Appears in Collections: | Chemical Sciences - Master's Theses Master's Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FINAL THESIS - Heather Cornthwaite - MSc Thesis 2014.pdf | 2.77 MB | Adobe PDF | View/Open |
Items in LU|ZONE|UL are protected by copyright, with all rights reserved, unless otherwise indicated.