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DC Field | Value | Language |
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dc.contributor.author | Alharbi, Albatul | - |
dc.date.accessioned | 2022-01-27T15:35:47Z | - |
dc.date.available | 2022-01-27T15:35:47Z | - |
dc.date.issued | 2021-12-16 | - |
dc.identifier.uri | https://zone.biblio.laurentian.ca/handle/10219/3826 | - |
dc.description.abstract | In traditional medicine, Ferula assafoetida (F. assafoetida), has been used as an antiseptic, anti-diabetic, anti-inflammatory, and anti-cancer agent. In recent years its anti-cancer and antiinflammatory activities have become a focus in drug research. We investigated the in vitro cytotoxicity and anti-inflammatory effects of ethanolic extracts of F. assafoetida and five known components (ferulic acid, vanillic acid, quercetin, ellagic acid, and p-coumaric acid) on a group of malignant and non-malignant breast cell lines and the THP-1 monocyte-like cell line. Our results showed that treatment with the ethanolic extract of F. assafoetida, and the components, had a significant effect on cell viability and apoptosis induction for the human MCF-7, MDA-MB-231, and murine 4T1 breast cancer cell lines compared to the non-malignant human HBL-100 breast cells. This research also showed that THP-1 peripheral blood monocytic leukemia cells, differentiated into macrophages, could be further polarized into the M1 inflammatory phenotype by treatment with extracts of F. assafoetida and the components. There was a significant increase in the expression of CD80, a marker associated with the M1 macrophage subtype, but no increase in expression of the M2 subtype marker, CD163, in treated cells. Further, this polarization of the THP-1-dependent macrophages showed an increased ability to damage MCF-7 or MDA-MB-231 cell monolayers in co-culture experiments. Therefore, treatment with F. assafoetida extracts can also indirectly cause the death of cancer cells via activation of immune cells. These results confirm that F. assafoetida is a potential source of anti-cancer and immune modulatory compounds and that further investigation is needed to reveal the mechanisms of F. assafoetida’s effects on apoptosis and immunomodulation. | en_US |
dc.language.iso | en | en_US |
dc.subject | Ferula assafoetida, | en_US |
dc.subject | anti-cancer, | en_US |
dc.subject | pro-inflammation, | en_US |
dc.subject | cytotoxicity | en_US |
dc.subject | apoptosis | en_US |
dc.subject | immunomodulation | en_US |
dc.subject | macrophage polarization | en_US |
dc.title | Cellular effects of Ferula Assafoetida on breast cancer cells and inflammatory responses in cultured monocytes | en_US |
dc.type | Thesis | en_US |
dc.description.degree | Doctor of Philosophy (PhD) in Biomolecular Sciences | en_US |
dc.publisher.grantor | Laurentian University of Sudbury | en_US |
Appears in Collections: | Biomolecular Sciences - Doctoral Theses |
Files in This Item:
File | Description | Size | Format | |
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Albatul Alharbi PhD Thesis Dec 16 corrected.pdf | 3.93 MB | Adobe PDF | View/Open |
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