Please use this identifier to cite or link to this item:
https://zone.biblio.laurentian.ca/handle/10219/2259
Title: | Energy-producing ability of bacteria under oxidative stress |
Authors: | Appanna, Varun P. |
Keywords: | Reactive nitrogen species (RNS);Pseudomonas fluorescens;fumarate;ATP |
Issue Date: | 7-Oct-2014 |
Publisher: | Laurentian University of Sudbury |
Abstract: | Nitrosative stress is caused by reactive nitrogen species (RNS) and is toxic to most organisms. RNS are generated by the immune system to combat infectious microbes and are known to impede O2-dependent energy production. The goal of this study was to elucidate alternative adenosine triphosphate (ATP)-forming pathways that enable the model bacterium Pseudomonas fluorescens to survive a nitrosative challenge in a fumarate medium. Fumarate was metabolized by fumarase C (FUM C), a RNS-resistant enzyme and fumarate reductase (FRD). The enhanced activities of pyruvate phosphate dikinase (PPDK), adenylated kinase (AK) and nucleoside diphosphate kinase (NDPK) provided an effective route to ATP production by substrate-level phosphorylation (SLP), a process that does not require O2. The metabolic networks utilized to neutralize nitrosative stress reveal potential target against RNS-tolerant bacteria and a route to the conversion of fumarate into succinate, a value-added product. |
URI: | https://zone.biblio.laurentian.ca/dspace/handle/10219/2259 |
Appears in Collections: | Biology - Master's Theses Master's Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
V. Appanna Masters Thesis FINAL.pdf | 1.37 MB | Adobe PDF | View/Open |
Items in LU|ZONE|UL are protected by copyright, with all rights reserved, unless otherwise indicated.